Journal: Frontiers in Immunology

Article Title: TLR3 Signaling Promotes the Induction of Unique Human BDCA-3 Dendritic Cell Populations

doi: 10.3389/fimmu.2016.00088

Figure Lengend Snippet: Poly I:C-stimulated ILT4 − and ILT4 + BDCA-3 cDCs have unique cytokine and gene signatures . (A) Experimental design of BDCA-3 DC phenotyping. (B) BDCA-3 cDCs were cultured with Poly I:C for 18 h and then sorted into ILT4 − and ILT4 + populations. Cells were then plated without further stimulation for 18 h. Supernatants were assayed for cytokine and chemokine content by luminex analysis. P -values generated using two-tailed student’s paired t -test (95% confidence interval). Graphs represent four donors. (C) BDCA-3 cDCs were stimulated with Poly I:C for 18 h, Golgistop was then added for 6 h. Cells were surface stained with ILT3, ILT4, and CD141 and then intracellularly stained with IFN-γ and TNF-α. Data showing intracellular staining are representative of one donor out of four. (D) Genomic profiling of ILT4 − vs. ILT4 + was performed using GeneChip Human Gene 1.0 ST arrays. Principal component analysis (PCA) was computed using OmicSoft ArrayStudio, and a plot was generated to show the relative clustering of ILT4 − and ILT4 + . ILT4 − and ILT4 + populations were compared to each other by t -test with a threshold set for a fold change >1.5 and a P -value <0.05. ILT4 gene expression was confirmed by qPCR. (Data shown are one representative donor out of four).

Article Snippet: A 3D plot generated by PCA with OmicSoft ArrayStudio across all probe sets revealed that ILT4 + cells are most dissimilar from ILT4 − cells (Figure D).

Techniques: Cell Culture, Luminex, Generated, Two Tailed Test, Staining, Gene Expression

Journal: Metabolites

Article Title: NMR-Based Tissular and Developmental Metabolomics of Tomato Fruit

doi: 10.3390/metabo9050093

Figure Lengend Snippet: PCA of absolute values of 39 metabolites and starch measured by proton nuclear magnetic resonance ( 1 H-NMR), liquid chromatography coupled with diode array detection (LC-DAD), or enzymatic analyses in five tomato fruit tissues and fruit without seeds at four stages of development, and expressed on a DW basis. 3D scores plot for PC1, PC2, and PC3. Exocarp in red, mesocarp with endocarp in orange, columella with placenta in yellow, locular tissue in light green, seeds in dark green, and fruit without seeds in light grey.

Article Snippet: The PCA 3D plot was done with XLSTAT (v2012.3.01, Addinsoft, Paris, France).

Techniques: Starch, Nuclear Magnetic Resonance, Liquid Chromatography